Click here for more information [http://eye.sbc28.com/m2?r=uDVhNjFiODI5Yjg1YjUzNmE5ZjRiZGVjMMQQ0KHQ0wzk0IlaTe7QnGzQlV9MVdC60I3EENDIRNC1f9CN0IVHdtCl0IvQzNCpWNDGCFDZJWZpd2FyZS1vcGVuY2FsbC1oZWxwQGxpc3RzLmZpLXdhcmUuZXWglsQHCONTACT_ILZ5RVMxZjQyRlIzYWxpOHlwV01ZSVVBxA4Q0MAIL_DQjkURAkzQg9CN_NCAoLFPUklHSU5fQ0FQVElPTl9JRLZMaXN0ZSBBY3RpZnMgMjAxNS0yMDE3xAo5EgYg0N_QyiDQ0P8gpExJU1TEDjxz0I0T8NCORRECTNCD0I380ICgxAZJMBMT8gOmTk9STUFM] [] Murine Antibodies [] | Rabbit Antibodies []| Immunoassays []| Therapeutic Ab [] Benefit from BIOTEM expertise at any steps of your project from ANTIBODY GENERATION to IMMUNOASSAY DEVELOPMENT… CONTACT US [info at biotem.fr ] … And enjoy a 15% DISCOUNT* on your ROYALTY-FREE TOOL for Research & Diagnostic STEP 1 – Immunization Several species: Mouse, Rat, Rabbit, etc. For each project, we define with our clients very precise specifications based on the • Target typology: membrane protein, peptide, small molecule, post-translational modifications, etc. • Final applications: ELISA, WB, FACS, IF, IHC, IP, functional assays, diagnostic test, etc. • Project specificity: Material available, specificity, deadline, etc. Depending on each project, BIOTEM proposes a large panel of strategies: • High affinity protocol, Rapid Antibody Development (R.A.D®), Small Size Immunogen (S.S.I®), Anti-Drug Antibody (ADA), etc... STEP 2 – Ab Screening Hybridoma & Phage Display Technology After the immunization step, BIOTEM implements several technologies for the identification of high affinity and specificity antibodies: • Hybridoma: Lymphocyte hybridization, Plating out of fused cells on 10 or 20 microtitre plates, screening and confirmation by ELISA, WB, IHC, etc. Learn more [] • Phage Display: Immune library construction (scFv), high throughput screening by Phage Display, sequencing. Learn more [] At the end of the STEP 2, the CLIENT has the possibility to test hybridoma supernatants or purified clones to select at early stage the best candidates. Selected candidates will be cloned or reformatted (scFv to Full Ig format). STEP 3 – Production (mg to g) Hybridoma / Recombinant Antibody Production BIOTEM offers different systems for the production and purification of antibodies including low endotoxin conditions, quality control (ISO 9001 & ISO 13485), etc. • Hybridoma: in vivo or in vitro production systems. Learn more [] • Recombinant Antibody: Fully integrative platform with CHO cell system (transient transfection). Learn more [] STEP 4 – Immunoassay ELISA & LFIA Development – IVD medical device BIOTEM can assist the CLIENT in the development of immunoassays (ELISA and Lateral Flow). Thanks to its ISO 13485 certification, BIOTEM can propose a fully complete program from the development to the IVD medical device production • ELISA: Principally used for R&D and for in vitro diagnostic, ELISA tests are enzymatic immunoassays for the detection and quantification of analytes in equipped laboratories. • Lateral Flow / Rapid Test: Lateral Flow tests (immunochromatography system assays) are based on the migration of nano or micro particles on strips. Many applications: Human health, Veterinary diagnostic, food industry, environment-quality evaluations, etc. Learn more [] [] [] [] *Find all our services on the website: www.biotem-antibody.com [] Offer valid until 31st March 2018 – Please mention the offer code LF/MAB15-2018to benefit from this discount – Conditions apply, please speak with our staff Tel.: +33 (0)4 76 65 10 91 Fax: +33 (0)4 76 67 48 86 info at biotem.fr [] [] If you do not wish to receive discount offers and/or information about our latest technologies please click here [http://eye.sbc28.com/r/USBSHOW/84/5a61b829b85b536a9f4bdec0/odMM5IlaTe6cbJVfTFW6jQ/yES1f42FR3ali8ypWMYIUA?email=fiware-opencall-help@lists.fi-ware.eu&adm=services@biotem-info.com] -------------- next part -------------- An HTML attachment was scrubbed... URL: <https://lists.fiware.org/private/fiware-opencall-help/attachments/20180305/1b49a371/attachment-0001.html>
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